Soluble and cellassociated transferrin receptor in lung. Measurement of circulating levels of the receptor are very useful in the diagnosis of iron deficiency in patients who also have anemia. Results we found that stfr and stfrfer were significantly higher at all sampling occasions compared with early. Pdf we evaluated the usefulness of soluble transferrin receptor stfr and of. Soluble transferrin receptor an overview sciencedirect topics. We found significant inverse correlations between serum concentrations of transferrin receptors and ferritin, the ratio of ferritin to aspartate aminotransferase, and. The serum concentration of stfr is directly proportional to the concentration of the receptor on the membrane. The soluble transferrin receptor stfr test is primarily ordered to help distinguish between anemia that is caused by iron deficiency and anemia that is caused by inflammation or a chronic illness socalled anemia of chronic disease or inflammation. Cystic fibrosis patients who frequently have inflammation or infections12 evaluating insulindependent diabetics who may have irondeficiency resulting from gastric. Response of soluble transferrin receptor and ironrelated pa.
Soluble transferrin receptor is a proteolytic product of the intact tfr and circulates in the blood as a monomeric fragment of the extracellular domain. Soluble transferrin receptor stfr conversion to nmoll, mg. Serum transferrin receptors are decreased in the presence of. Fifty consecutive newly diagnosed anaemic hb sep 08, 2016. It is not a routine test but may be ordered if other tests that evaluate the amount of iron. Soluble transferrin receptors are proteins found in blood that can be elevated with iron deficiency.
Levels of this receptor are increased in irondeficiency anemia. Pdf soluble transferrin receptor in complicated anemia. In general, to increase sensitivity and specificity, the measurement of serum soluble transferrin receptor should be performed in combination with other tests of iron status, including ferritin, tibc, and serum iron refer to. Jun 02, 2017 transferrin receptor tfr is a carrier protein for transferrin. Pdf the usefulness of soluble transferrin receptor stfr in. Is soluble transferrin receptor a good marker of iron.
The soluble transferrin receptor stfr immunoassay should not be used for the routine clinical evaluation of patients for iron status when ferritin immunoassay ferr ferritin, serum would be appropriate, such as in the absence of confounding pathologies inflammation, infection, chronic disease, or. Tfr binds diferric transferrin, and the receptortransferrin complex is internalized into an endosome, where the iron is transferred to the cytosol. Comparative response of epo and soluble transferrin receptor. Soluble transferrin receptor conventionally refers to the cleaved extracellular portion of transferrin receptor 1 that is released into serum. Soluble transferrin receptor, human, in vitro, antibody cat. Recombinant soluble transferrin receptor rstfr nibsc. Serum transferrin receptors test reference range transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency. A proteolytic product of the transferrin receptor circulates in plasma as soluble transferrin receptor stfr. Soluble serum transferrin receptor and transferrin receptoraferritin. Jurkat human t cell lymphoblastlike cell line whole cell lysate lane 3. Soluble transferrin receptors stfr are proteins found in blood that can be elevated with iron deficiency. After adding the product to the cart by clicking the button below you will see if there is a volume discount available. Serum transferrin receptors test reference range htq.
After recycling to the cell surface, the apotransferrin dissociates, and the receptor is free to repeat the process. Rapid diagnostics for pointofcare quantification of. Soluble transferrin receptor stfr, one of the main regulators of cellular iron homeostasis, is the truncated form of the tissue receptor that is encoded by the human tfr gene chromosome 3. In recent years, soluble transferrin receptor stfr has been introduced as a sensitive, early and highly quantitative new marker of iron depletion, increasing in proportion to tissue iron deficit. The existence of a receptor for transferrin iron uptake had been recognized over half a century back. Soluble transferrin receptors and iron deficiency, a step.
Data were evaluated using analysis of variance for repeated measures as the women continuing the study were found to be representative of those entering the study. Transferrin receptor tfr is a carrier protein for transferrin. Comparative response of epo and soluble transferrin. Analytical and clinical implications of soluble transferrin. Soluble transferrin receptor arup lab test directory. Determination of serum soluble transferrin receptor stfr is proposed to. Serum ferritin, transferrin and soluble transferrin receptor. Soluble transferrin receptorferritin index in the evaluation of anemia. Plasma soluble transferrin receptor assay when screening for. This study investigated the response of stfr during highaltitude exposure in human and verified that stfr was related to epo response in this case. The level of soluble transferrin receptors is directly proportional to the total mass of tissue receptors 2. Now also available on your iphone, ipad, and android. Soluble transferrin receptor log ferritin tfrf index, using a cutoff of 5. Serum levels of ferritin, transferrin saturation and stfr were analyzed in all patients and controls.
Soluble transferrin receptor levels may be helpful in distinguishing iron deficiency from anemia of chronic inflammation in cf because, unlike serum ferritin and transferrin, soluble transferrin receptor does not appear to be an acutephase reactant. Because tfr is predominantly expressed by erythroid progenitor cells, the main sources of stfr are erythropoietic cells of the bone marrow including circulating reticulocytes. Thus, the concentration of stfr in plasma or serum is an indirect measure of total tfr. Soluble transferrin receptor pointofcare testing portable diagnostics 1. Release of the soluble transferrin receptor is directly. Immunological study of complex formation between soluble. Biological and clinical aspects of soluble transferrin. Clinical utility of the soluble transferrin receptor and. Soluble transferrin receptors stfr are proteins found in blood that can become elevated in the presence of irondeficiency.
Serum ferritin, transferrin and soluble transferrin receptor levels in multiple sclerosis patients constantinos sfagos, alexandros c makis, aristeidis chaidos, eleftheria c hatzimichael, androniki dalamaga, katerina kosma, and konstantinos l. Pdf measurement of soluble transferrin receptor in serum of. This receptor is a protein dimer of two identical subunits, linked together by two pairs of disulfide bonds. Soluble tfr is a truncated monomer of tissue receptor, lacking its first 100 amino. This test measures the amount of soluble transferrin receptors within the blood, to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anaemia. To assess the effect of shortterm iron supplementation on soluble transferrin receptor stfr and the soluble transferrin receptor log ferritin ratio in a group of elite, irondepleted, nonanemic female athletes and to assess the relationship between soluble transferrin receptor and serum ferritin in a group of elite, irondepleted female athletes. It imports iron by internalizing the transferrin iron complex through receptor mediated endocytosis. Soluble serum transferrin receptor and transferrin receptor.
Serum soluble transferrin receptor increases in iron deficiency and is usually unaffected by chronic disease states. Hepcidin, soluble transferrin receptor and il6 levels in. Soluble transferrin receptor test detail quest diagnostics. In plasma, the soluble transferrin receptor is present in the form of a complex with transferrin having a molecular weight of approximately 320 kd. The transferrin receptor is comprised of two identical subunits, each composed of 760 aminoacids with a molecular mass of 95 kdaltons. The serum concentration of stfr is directly proportional to the. Using soluble transferrin receptor and taking inflammation. Soluble transferrin receptor, human, in vitro, antibody.
Soluble serum transferrin receptor and transferrin. Soluble transferrin receptors and iron deficiency 347 the endosomes acidosomes, and the tftfr complex is returned back to the cell surface, where apotransferrin is released again 41. Rapid diagnostics for pointofcare quantification of soluble. Feb 12, 2014 transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency. Soluble transferrin receptor stfr conversion to nmoll. Use of multiple antibiotics in patients with cf is routine. Soluble transferrin receptors in serum are found as a truncated fragment of transmembrane transferrin receptors 3, 4. Transferrin receptor an overview sciencedirect topics.
Soluble transferrin receptor stfr concentrations are inversely related to iron status. Soluble transferrin receptor arises from proteolysis of the intact protein on the cell surface, leading to monomers that can be measured in plasma and serum. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anemia. Transferrin receptor tfr expression is regulated by the iron regulatory protein system. Demonstration of an epitope of the transferrin receptor in human cervical epitheliuma potentially useful cell marker. Pdf biological and clinical aspects of soluble transferrin. Soluble transferrin receptors and iron deficiency 347 the endosomes acidosomes, and the tftfr complex is returned back to the cell surface, where apo transferrin is released again 41. Fifty consecutive newly diagnosed anaemic hb soluble and placental transferrin receptors as standards for the determination of soluble transferrin receptor in humans. This test measures the amount of soluble transferrin receptors within the blood, to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anaemia iron is an essential nutrient absorbed from the diet. Transferrin receptor wikimili, the free encyclopedia. Pdf the concentration of soluble transferrin receptor stfr in serum is reported to be useful.
The soluble transferrin receptor stfr immunoassay should not be used for the routine clinical evaluation of patients for iron status when ferritin immunoassay ferr ferritin, serum would be appropriate, such as in the absence of confounding pathologies inflammation, infection, chronic disease, or malignancy. To evaluate the effect of different types and volumes of physical activity on stfr concentration, variables of iron status ferritin. Serum transferrin receptor and its ratio to serum ferritin in. Virtually all cells have transferrin receptors on their surface, but in the normal adult, about 80% of them are in the erythroid marrow. Role of soluble transferrin receptor and transferrin receptor. Soluble transferrin receptor and ferritin concentrations were nephelometrically determined by n latex reagent sets bade behring according to the manufacturers instructions after storage of the centrifuged 2600g. The amount of transferrin receptor expressed on a cell is proportional to the cells need for iron. Measurement of soluble transferrin receptor in serum of. Soluble and cellassociated transferrin receptor in lung cancer. However, the specific effect of altitudeinduced erythropoiesis on stfr remains poorly documented.
Soluble transferrin receptor an overview sciencedirect. It may be ordered as an alternative to ferritin when you have a chronic illness andor inflammation is present or suspected. Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of irondeficiency anemia. It has been included in multivariable blood testing models for the detection of performance enhancing erythropoietin misuse in sport. Effects of exercise on soluble transferrin receptor and. Soluble transferrin receptor stfr test test results. A soluble form of the tfr stfr has been identified in animal and human serum. Soluble transferrin receptor and erythrocyte protoporphyrin testing. Soluble transferrin receptor stfr useful for evaluation of suspected iron deficiency in patients who may have inflammation, infection, or chronic disease and other conditions in which ferritin concentration does not correlate with iron status, including. Serum transferrin receptor level is not altered in invasive adenocarcinoma of the breast. The soluble transferrin receptor stfr, a truncated form of the membraneassociated transferrin receptor, has been reported to be a sensitive indicator of iron deficiency and is not an acutephase reactant. The soluble transferrin receptor test is not routine but may be ordered along with or in follow up to other iron tests when you have a normal or nearnormal ferritin level but iron deficiency is still suspected. Iron is an essential nutrient absorbed from the diet.
The uptake of iron by cells is mediated by a cellsurface transferrin receptor tfr. In order to improve the diagnostic accuracy of ferritin or stfr as a standalone marker, the normal cutoff value needed to be increased by 810% and 83%. For patients receiv ing iron supplements, the time free from iron therapy before blood sam pling was 36 days. Soluble transferrin receptor soluble transferrin receptor stfr values can be within normal limits over a broad range of body iron stores and is elevated only when there is functional i. Proteolysis leads to the soluble form of the transferrin receptor stfr. Plasma soluble transferrin receptor assay when screening. The transferrin receptor tfr is a homodimeric type ii membrane protein that mediates iron uptake into the cells. Effects of exercise on soluble transferrin receptor and other.
Soluble transferrin receptor stfr is a new marker of iron status and erythropoietic activity. Hela human epithelial carcinoma cell line whole cell lysate lane 2. It is not a routine test but may be ordered if other tests that evaluate the amount of iron in the body do not provide conclusive information. The aim of this study was to evaluate a possible role for the soluble transferrin receptor assay when screening for iron.
Serum ferritin vs transferrin receptorferritin index. Since most of the tissue receptors can be found on. Serum ferritin, transferrin and soluble transferrin. It may be ordered as an alternative to ferritin when you have a. Soluble transferrin receptor stfr, serum billings clinic. To test the hypothesis that the quantities of circulating transferrin receptors are reduced in iron overload, we studied serum transferrin receptors and indirect measures of iron status in 150 subjects from rural zimbabwe.
Iron deficiency anemia low transferrin saturation, and a high total ironbinding capacity. Soluble transferrin receptor stfr is an extracellular part of the tfr molecule that is truncated from the. It imports iron by internalizing the transferriniron complex through receptormediated endocytosis. Serum transferrin receptor levels for the assessment of iron status. The prevalence of iron depletion, iron deficient erythropoiesis ide, and iron deficiency anemia ida was assessed in preschool inuit children using soluble transferrin receptor stfr and traditional indicators of iron status while disregarding or taking inflammation into account when defining sf cutoffs. Epo, stfr, red cell volume rcv, ferritin, and iron intake were measured during. Soluble transferrin receptor for the evaluation of. Evaluation of suspected iron deficiency in patients who may have inflammation, infection, or chronic disease and other conditions in which ferritin concentration does not correlate with iron status, including. Soluble transferrin receptor stfr and stfrlog ferritin index for. Soluble transferrin receptor stfr, serum barnesjewish.
New cutoff values for ferritin and soluble transferrin. Soluble transferrin receptor article about soluble. Transferrin receptor is a nonrtk constitutively recycling receptor and thus can be used as a control to assess rtk specificity of met trafficking. Circulating soluble transferrin receptor according to. It is needed for the import of iron into the cell and is regulated in response to intracellular iron concentration.
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